Comparison of neutralizing antibody titers (NT) to SARS-CoV-2 vaccine strain (WA-1), Beta, Delta, and Omicron VOCs from health care workers with paired serum samples in a longitudinal cohort. Data are shown for three time points: within 14 to 44 days after dose 2 (time point 1), at least 8 months after dose 2 (time point 2), and within 14 to 44 post-boost (time point 3). The top panel shows the NT titer for each variant across the three time points with connecting lines and illustrates 15 paired samples from time points 1 and 3. The bottom panel shows the NT at each time point for each VOC. The fold change (increase/difference) represents the geometric median fold change. P values have been corrected for multiple comparisons using the Bonferroni method.