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Amplification curves for simultaneous detection of multiple carbapenemase genes using on-chip LAMP. (A) ATCC25922, negative control for carbapenemase genes; (B) E. coli, blaNDM; (C) E. coli, blaKPC; (D) A. baumannii, blaOXA-23; (E) K. pneumoniae, blaIMP; (F) K. pneumoniae, blaVIM; (G) P. aeruginosa, blaKPC+ blaoprD2; (H) K. pneumoniae, blaOXA-48; (I) A. baumannii, blaOXA-58. The template used was gDNA extracted from clinical bacterial isolates with concentrations of 50 ng/μL.
