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. 2022 Oct 13;23(20):12188. doi: 10.3390/ijms232012188

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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YESS–PSSC system for quantitatively characterizing Ulp1–Smt3 specificity. (A) Scheme of YESS–PSSC system for substrate specificity analysis of Ulp1 against Smt3. Left: represents cells merely expressing substrate part (Aga2-FLAG-Smt3-HA). Right: represents cells simultaneously expressing Ulp1 and substrate part (Aga2-FLAG-Smt3-HA). (B) Flow cytometry analysis that characterizes Ulp1–Smt3 specificity in YESS–PSSC system. Left: HZ1 corresponded to the result of (A) left. Right: HZ2 corresponded to the result of (A) right.