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Mapping residue specificity of Ulp1 at Smt3 P1’ position. Ulp1-GAL1-GAL10-Aga2-FLAG-Smt3GG↓X-HA (X is an arbitrary amino) cassettes are, respectively, expressed in YESS–PSSC system, followed by direct flow cytometry analysis. (A) Representative flow cytometry results. (B) Data of A are presented as average cleavage efficiency (n = 3 independent experiments).
