TABLE 1.
SEN and SPE of IFA using different sources of granulocytic ehrlichial antigena
| Antigen source | Antibody tested and cutoff titer | No. of:
|
SEN | No. of:
|
SPC | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TP | FN | TN | BD | MSF | AQF | BE | EBV | CMV | CQF | SLE | Total | ||||
| PA | IgG or IgM > 32 | 25 | 5 | 0.833 | 127 | 2 | 1 | 0 | 7 | 0 | 0 | 0 | 0 | 10 | 0.927 |
| IgG > 32 | 24 | 6 | 0.800 | 127 | 2 | 1 | 0 | 7 | 0 | 0 | 0 | 0 | 10 | 0.927 | |
| IgG > 16 | 25 | 5 | 0.833 | 121 | 8 | 1 | 0 | 7 | 0 | 0 | 0 | 0 | 16 | 0.883 | |
| IgG > 8 | 25 | 5 | 0.833 | 120 | 9 | 1 | 0 | 7 | 0 | 0 | 0 | 0 | 17 | 0.875 | |
| IgM > 32 | 8 | 22 | 0.266 | 137 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | |
| IgM > 16 | 12 | 18 | 0.400 | 129 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 0.941 | |
| IgM > 8 | 19 | 11 | 0.633 | 117 | 20 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 20 | 0.854 | |
| IC | IgG or IgM > 32 | 20 | 10 | 0.666** | 124 | 3 | 0 | 0 | 6 | 0 | 1 | 2 | 1 | 13 | 0.905 |
| IgG > 32 | 17 | 13 | 0.566* | 124 | 3 | 0 | 0 | 6 | 0 | 1 | 2 | 1 | 13 | 0.905 | |
| IgG > 16 | 23 | 7 | 0.766 | 121 | 6 | 0 | 0 | 6 | 0 | 1 | 2 | 1 | 16 | 0.883 | |
| IgG > 8 | 23 | 7 | 0.766 | 117 | 10 | 0 | 0 | 6 | 0 | 1 | 2 | 1 | 20 | 0.854 | |
| IgM > 32 | 11 | 19 | 0.366 | 136 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0.992 | |
| IgM > 16 | 17 | 13 | 0.566 | 131 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 6 | 0.956 | |
| IgM > 8 | 20 | 10 | 0.666 | 121 | 15 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 16 | 0.883 | |
| MRL | IgG > 32 or IgM > 20 | 28 | 2 | 0.933** | 126 | 2 | 1 | 1 | 7 | 0 | 0 | 0 | 0 | 11 | 0.919 |
| IgG > 32 | 26 | 4 | 0.866* | 127 | 1 | 1 | 1 | 7 | 0 | 0 | 0 | 0 | 10 | 0.927 | |
| IgG > 16 | 27 | 3 | 0.900 | 123 | 5 | 1 | 1 | 7 | 0 | 0 | 0 | 0 | 14 | 0.897 | |
| IgG > 8 | 27 | 3 | 0.900 | 116 | 12 | 1 | 1 | 7 | 0 | 0 | 0 | 0 | 21 | 0.846 | |
| IgM > 20 | 10 | 20 | 0.333 | 135 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0.985 | |
a
Sera used were from 30 proven HGE cases and 137 control sera from Blood Donors (BD) (n = 96), Mediterranean (MSF) spotted fever (n = 10) (AQF) acute Q fever (n = 10), Bartonella endocarditis (BE) (n = 10), Epstein-Barr virus (EBV) (n = 2), cytomegalovirus (CMV) (n = 3), (CQF) Chronic Q fever (n = 3), and systemic lupus erythematosus (SLE) (n = 3). Symbols: *, Difference is significant with P = 0.0098233 (chi-square test); **, Difference is significant with P = 0.0099243 (chi-square test). Abbreviations: TP, true positive; TN, true negative; FP, false positive; FN, false negative.