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. 2022 Oct 11;23(20):12059. doi: 10.3390/ijms232012059

Figure 3.

Figure 3

(A,B) SH-SY5Y cell adhesion and proliferation on different OxPVA/ChS-based scaffolds, evaluated by MTT assay. Bioactivation occurred through the incorporation of biochemical stimuli (+EAK, +EAK-IKVAV, and +EAK-YIGSR) within the chitosan layer. (A) At 72 h from seeding, no statistically significant difference in cell adhesion was observed comparing groups. Differences in proliferation were detected since day 7. (B) The higher total cells number was displayed by OxPVA/ChS+EAK scaffolds, while the lower cells number was observed on OxPVA/ChS+EAK-YIGSR scaffolds (* p < 0.05; ** p < 0.01). (C–J) OxPVA/ChS-based scaffolds surface analysed by SEM at 72 h (C–F) and 7 days (G–J) from SH-SY5Y cell seeding. Specifically, the experimental groups included: OxPVA/ChS (C,G); OxPVA/ChS+EAK (D,H); OxPVA/ChS+EAK-IKVAV (E,I); and OxPVA/ChS+EAK-YIGSR scaffolds (F,J). Scaffolds surface ultrastructure was not distinguishable once colonized by cells (white arrows). Representative clumps with roundish cells are shown (white asterisk). Scale bar: 20 μm.