Table 2.
DON production in the WT and ∆FpPEX11 strains.
DON Production | Relative Expression Levels of TRI | ||||
---|---|---|---|---|---|
ELISA (μg/mg) | HPLC (μg/g) * | TRI5 | TRI6 | TRI10 | |
WT | 133.78 ± 18.36 a | 14.59 ± 3.47 b | 1.00 ± 0.09 | 1.00 ± 0.22 | 1.00 ± 0.22 |
∆FpPEX11a | 0 | 0.16 ± 0.01 c | 0.11 ± 0.04 | 0.16 ± 0.06 | 0.19 ± 0.02 |
∆FpPEX11b | 0 | 0.00763 ± 0 c | 0.13 ± 0.02 | 0.10 ± 0.02 | 0.15 ± 0.08 |
∆FpPEX11c | 121.43 ± 21.65 a | 32.05 ± 6.00 a | 7.198 ± 0.09 | 1.82 ± 0.24 | 0.90 ± 0.21 |
DON production was analyzed by HPLC–MS/MS (HPLC) and Deoxynivalenol (DON) Rapid Test Kit (ELISA). * Logarithmic transformation of DON production by HPLC and the different letter on the bars for each treatment indicate significant difference at p < 0.05 by Duncan’s multiple range test.