FIG 5.

Profiling of UPEC main virulence genes expression from the gut reservoir to urothelium. (A) Heatmap displaying the log₂ fold change UPEC virulence genes expression according to the ecosystem exposure (colonic dialysate, planktonic and adhered bacteria to UC), the microbial metabolome origin and the treatment. Induction (log₂ fold change expression ≥ 1) is denoted in shade of red, and repression (≤ -1) in shade of green, as determined by RT-qPCR. Samples with failed amplification are displayed in gray. (B) Selection of genes from dialysis cassettes remaining significantly different between control and PAC conditions are indicated with P ≤ 0.05 (*) or P ≤ 0.01 (**), as determined by the Friedman post hoc Wilcoxon test. (C) Selection of genes from adhered UPEC treated with PAC that are significantly different between donors A and B, as determined by the Friedman post hoc Wilcoxon test.