TABLE 4.
Detection of FPV and REV and humoral antibody response to REV in the chickens, vaccinated or not after infection with field FPV strains
| Test seta | Challenge strain | Routeb | FPV detection in skin lesion |
FPV detection in trachea |
REV detection in thymus |
Anti-REV antibody positive |
||||
|---|---|---|---|---|---|---|---|---|---|---|
| 1 wpi† | 4 wpi | 1 wpi | 4 wpi | 1 wpi | 4 wpi | 2 wpi | 4 wpi | |||
| A | PBS | ID | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/9 | 0/6 |
| A | 10D392 | ID | 1/3 | 0/3 | 0/3 | 0/3 | 3/3 | 1/3 | 9/9 | 7/7 |
| A, C | 19D64 | ID | 1/3 | 3/3 | 0/3 | 0/3 | 0/3 | 1/3 | 7/9 | 9/9 |
| A, B | 18R59 | ID | 1/3 | 3/3 | 2/3 | 3/3 | 2/3 | 1/3 | 7/9 | 8/8 |
| B | 18R59 | IV | 2/3 | 2/3 | 3/3 | 3/3 | 3/3 | 0/3 | 9/9 | 3/3 |
| B | 18R59 | IN | 0/3 | 1/2 | 0/3 | 2/2 | 0/3 | 0/2 | 5/7 | 2/2 |
| C | 18R59 after V-kr vaccination | IN | 0/3 | 0/3 | ntc | 0/3 | 1/3 | nt | 0/9 | 2/6 |
| C | 19D64 after V-cmp vaccination | ID | 0/3 | 0/3 | 0/3 | 0/3 | 3/3 | 0/3 | 6/6 | 6/6 |
| C | 19D64 after V-blen vaccination | ID | 0/3 | 0/3 | 0/3 | 0/3 | 2/3 | 0/3 | 6/6 | 1/6 |
a
A test was performed to compare pathogenicity between different strains; B test to compare pathogenicity between different inoculation routes; C test to evaluate vaccine efficacy.
b
ID, intradermal; IN, intranasal; IV, intravenous; WPI, weeks postinoculation.
c
nt, not tested.