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. 2022 Oct 14;23(20):12249. doi: 10.3390/ijms232012249

Figure 2.

Figure 2

High-dose vitamin C enhances the caspase-independent apoptosis response of PDAC cells. (A) PDAC cells were treated with vitamin C (4 or 5 mM) for 24 h and assayed for apoptotic cell death by annexin V/PI staining and flow cytometry. (B) PDAC cells were treated with vitamin C (4 or 5 mM) for 24 h and immunoblotted with the indicated antibodies. Error bars with the mean ± s.d. of three independent experiments. (C) PDAC cells were treated with vitamin C (4 or 5 mM) for 24 h with or without zVAD-fmk (50 μM) and assayed for apoptotic cell death by annexin V/PI staining and flow cytometry. Error bars represent the s.d. of triplicate wells from representative experiments; ** p < 0.01. NS, not significant.