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. 2022 Oct 14;23(20):12249. doi: 10.3390/ijms232012249

Figure 4.

Figure 4

High-dose vitamin C suppresses the invasiveness and metastasis of PDAC cells. (A) PDAC cells were treated with vitamin C (4 or 5 mM) for 24 h and immunoblotted with the indicated antibodies. Error bars with the mean ± s.d. of three independent experiments. (B) Migration assay for 8988T cells loaded into the upper chamber of a 0.4% gelatin-coated transwell and treated with vitamin C (4 or 5 mM) for 24 h in the lower chamber. The bar plot represents the percentage of cell migration for each panel. (C) Invasion assay for 8988T cells seeded into the upper chamber of a Matrigel (1 mg/mL) coated transwell and treated with vitamin C (4 or 5 mM) for 24 h in the lower chamber. The bar plot represents the percentage of cell invasion for each panel. (D) PDAC cells were treated with vitamin C (4 or 5 mM) for 24 h and immunoblotted with the indicated antibodies. Error bars with the mean ± s.d. of three independent experiments. (E) PDAC cells expressing FLAG-Snail 6SA were treated with vitamin C (5 mM) for 24 h and immunoblotted with the indicated antibodies. Error bars reflect the s.d. of three independent experiments; * p < 0.05, ** p < 0.01.