Skip to main content
. 2022 Oct 14;23(20):12249. doi: 10.3390/ijms232012249

Figure 5.

Figure 5

Effects of vitamin C on the migration ability of PDAC cells. (A) TOP/FOP luciferase reporter assays of 8988T cells treated with vitamin C (4 or 5 mM) for 24 h. (B,C) PDAC cells were treated with vitamin C (4 or 5 mM) for 24 h and immunoblotted with the indicated antibodies. Error bars reflect the s.d. of three independent experiments. (D,E) PDAC cells were treated with vitamin C (5 mM) for 24 h with or without CHIR and immunoblotted against the indicated antibodies. Invasion assay for PDAC cells seeded into the upper chamber of a Matrigel (1 mg/mL) coated transwell and treated with vitamin C (5 mM) for 24 h in the lower chamber. The bar plot represents the percentage of cell invasion for each panel. (F) Invasion assay for PDAC cells seeded into the upper chamber of a Matrigel (1 mg/mL) coated transwell and treated with vitamin C (5 mM) for 24 h in the lower chamber. Error bars represent the s.d. of triplicate wells from a representative experiment among three independent experiments; ** p < 0.01. CHIR, CHIR99021.