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. 2022 Oct 20;23(20):12574. doi: 10.3390/ijms232012574

Figure 2.

Figure 2

Downregulation of PCAF inhibits AngII-induced senescence of ECs. (A,B) Western blot to determine the expression of PCAF in each group (n = 6). (C,D) β-galactosidase staining to detect senescence of ECs; Senescent VSMCs were marked in blue, the scale bar is 100 μm, 400× (n = 5). (E,F) p53, p21, p16, ICAM-1 and VCAM-1 protein expression (n = 6). (G) Detection of PCAF (green) and p21 (red) levels by immunofluorescence double staining; the scale bar is 100 μm, 400× (n = 3). ** p < 0.01 vs. the control group; # p < 0.05, ## p < 0.01 vs. the AngII induction with Ad-GFP group (AngII + Ad-GFP group).