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. 2022 Oct 20;23(20):12574. doi: 10.3390/ijms232012574

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Nrf2/HO-1 mediates the anti-senescence effect of PCAF downregulation. (A) ECs cells were lysed for immunoprecipitation (IP) assays, and PCAF or Nrf2 were detected by Western blot in the precipitates pulled down by antibody against PCAF or normal IgG. Input indicates the cellular lysate before adding antibodies (n = 3). (B,C) The total expression of Nrf2 and the expression of Nrf2 in nucleus, detected by western blot (n = 6). (D) Immunofluorescence to detect the distribution of Nrf2 (green); arrows represent nuclear translocation of Nrf2; the scale bar is 50 μm, 400× (n = 3). (E,F) HO-1 and NQO-1 protein expression (n = 6). ** p < 0.01 vs. the control group; # p < 0.05, ## p < 0.01 vs. AngII + Ad-GFP group.