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Comparison of HUDSON-RT-RAA-CRISPR/Cas13a, nested PCR and antigen ELISA in testing 50 spleen samplesa
| Method | Positive (+) | Suspected (±) | Negative (−) |
|---|---|---|---|
| HUDSON-RT-RAA-CRISPR/Cas13a | 15 | 0 | 35 |
| Nested PCR | 15 | 0 | 35 |
| Antigen ELISA | 6 | 4 | 40 |
ELISA, enzyme-linked immunosorbent assay; HUDSON, heating unextracted diagnostic samples to obliterate nucleases; RT-RAA, reverse transcription recombinase-aided amplification.
