Skip to main content
. 2022 Oct 21;23(20):12676. doi: 10.3390/ijms232012676

Figure 1.

Figure 1

Description of the iMLS reporter and assay setup. (A) Mitophagy reporter description. (B) Representation and quantification of mitophagy modulations in U2OS-iMLS cells treated with DFP for 24 h. BafA1 was added for the last 2 h. (C) Representation and quantification of mitophagy modulations in U2OS-iMLS-PRKN cells treated with FCCP for 24 h. BafA1 was added for the last 2 h. Data represent the mean ± SEM of three experiments (significance was determined by one-way ANOVA followed by Dunnett’s multiple comparisons test where * p < 0.05 and *** p < 0.001 compared with control and # p < 0.05 and **** p < 0.0001 compared with DFP or FCCP, respectively). Scale bar = 50 µm.