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. 2022 Oct 21;23(20):12676. doi: 10.3390/ijms232012676

Figure 7.

Figure 7

IGS2.7 restored the autophagy flux in cellular and animal TARDBP models of ALS. (A) Lymphoblasts were incubated in the presence and absence of 5 µM IGS2.7 for 24 h. Hydroxychloroquine (HCQ) was added during the last 3 h when indicated. Each point represents the mean of three independent experiments. Data represent the mean ± SEM of three controls and four TARDBP lymphoblastic cell lines (significance was determined by one-way ANOVA followed by Dunnett’s multiple comparisons test, where *** p  <  0.001 vs. control and #### p < 0.001 vs. SOD1-ALS). (B) Histological samples of the anterior horn of the spinal cord of TDP-43 mice and wild-type controls. Tissues were immunostained for p62 and TOMM20 and quantified per MN. Data represent mean ± SEM of four different animals (significance was determined by one-way ANOVA followed by Dunnett’s multiple comparison test, where * p  <  0.05 compared with WT and # p  <  0.05 compared with TDP-Veh). Scale bar = 30 µm.