Figure 1.

Downregulation of m⁶A and METTL3 levels in psoriatic skin lesions. (A) The m⁶A levels of total RNAs in skin samples (n = 20) derived from patients with psoriasis vulgaris and healthy controls. (B) Expression of METTL3, METTL14, WTAP, FTO, and ALKBH5 in skin samples (n = 20) derived from patients with psoriasis vulgaris and healthy controls. (C) Correlation of m⁶A levels of total RNAs in human psoriatic skin (n = 20) with PASI scores. (D,E) Correlation of human m⁶A levels of total RNAs (D, n = 20) or expression of METTL3 (E, n = 20) in human psoriatic skin with PASI scores. (F) Expression of Mettl3 in skin samples from untreated mice (n = 6) and IMQ-induced mice (n = 6). (G) Immunohistochemical analysis was performed on mouse skin treated with vehicle (Ctr, n = 6) or IMQ (IMQ, n = 6) using anti-METTL3 monoclonal antibodies. Dark brown color indicates Mettl3 expression. Scale bars: 100 μm. (H) Expression of Mettl3 in the epidermis and dermis of skin samples from untreated controls (n = 6) or IMQ-treated mice (n = 6). Data represent the mean ± SEM. * p < 0.05, ** p < 0.01, NS, not significant.Circle, point, triangle and square represent each sample. Data represent the mean ± SEM. Two-tailed Mann–Whitney test (A,B), two-tailed unpaired Student’s t test (F,H), and Spearman’s r test (C,D,E) were used.