Skip to main content
. 2022 Oct 21;23(20):12657. doi: 10.3390/ijms232012657

Figure 6.

Figure 6

Hit compound validation in TMEM16A KO nasal organoids. (A) Brightfield images showing intrinsic lumen formation, without any stimulation, in both control and TMEM16A KO nasal organoids (G542X/CFTRdele2.3(21kb)); (B) quantification of organoid lumen size in control and knockout organoids (n = 3 independent donors); (C) validation of hit compounds on nasal organoid swelling in TMEM16A KO and control organoids (n = 3 independent donors, 2–6 measurements per donor); (D) representative confocal images (G542X/CFTRdele2.3 (21 kb)) of TMEM16A KO and control nasal organoids, stimulated with Terbutaline Sulfate (3 µM) as example of one of the hit compounds; (E) quantification of nasal organoid swelling after stimulation with Terbutaline Sulfate (3 µM) in TMEM16A KO and control organoids (n = 3 independent donors). Analysis of difference was performed with a paired (B) or unpaired (C) t-test. No significant results were found. Ns = non-significant.