FIG 5.

Virulence phenotypes of P. aeruginosa clinical isolates evolved under ciprofloxacin risk concentration. Relative quantification of different phenotypes with relevance for the virulence of P. aeruginosa, measured in P. aeruginosa PA14 and 10 clinical isolate populations evolved under 0.04 μg/mL of ciprofloxacin for 3 days, with respect to the ones evolved in LBB for the same interval (dotted line). The graph presents elastase activity (a), pyocyanin production (b), and biofilm formation (c). Error bars indicate standard deviations from three independent experiments, and statistically significant differences were assessed using the Student’s t test and are shown (*, P < 0.05; **, P < 0.005).