Table 1.
Summary of specific mutations in A/Singapore/G2-31/2014 following five serial passages in MDCK-SIAT1 and chick embryo fibroblast (CEF) cells. The dash symbol (–) indicates absence of specific mutations. N.D. denotes the passages in which the full-length gene could not be amplified.
| HA | NS1 | NA | PB1 | |||||
|---|---|---|---|---|---|---|---|---|
| Virus Passage | MDCK-SIAT1 | CEF | MDCK-SIAT1 | CEF | MDCK-SIAT1 | CEF | MDCK-SIAT1 | CEF |
| 1 | – | – | – | – | – | N.D. | – | N.D. |
| 2 | – | N.D. | – | N.D. | – | N.D. | – | N.D. |
| 3 | – | N.D. | – | N.D. | A736G (silent) | N.D. | A275C (Q84P) | N.D. |
| G325T (G101C) | ||||||||
| 4 | – | N.D. | – | N.D. | – | N.D. | – | N.D. |
| 5 | A1399G (D457G) | N.D. | – | N.D. | – | N.D. | – | N.D. |