Table 3.

List of PCR primers (and their sequences) used in this study.

Code Name	Primer Sequence (5′-3′)	Purpose
HA1F	AGCAGGGGATATTTTTATTAACC	Forward primer for preliminary HA1 screening
HA1R	CAACCATCCACCATTCCCTC	Reverse primer for preliminary HA1 screening
M1-M2F	CATCCTGTTGTATATGAGGCCCAT	Forward primer for real-time qPCR
M1-M2R	GGACTGCAGCGTTAGACGCTT	Reverse primer for real-time qPCR
NS1-M13F	TGTAAAACGACGGCCAGTAGCAAAAGCAGGGTGACAAAGACA	Forward primer for NS1 amplification
NS1-M13R	CAGGAAACAGCTATGACCAGTAGAAACAAGGGTGTTTTTTAT	Reverse primer for NS1 amplification
HA-F1-M13F	TGTAAAACGACGGCCAGTAAAGCAGGGGATAATTCTA	Forward primer for HA Fragment 1
HA-F1-M13R	CAGGAAACAGCTATGACCATTGCTGCTTGAGTGCTT	Reverse primer for HA Fragment 1
HA-F2-M13F	TGTAAAACGACGGCCAGTGGTTACTTCAAAATAC	Forward primer for HA Fragment 2
HA-F2-M13R	CAGGAAACAGCTATGACCAGTAGAAACAAGGGTGTTTT	Reverse primer for HA Fragment 2
NA-F1-M13F	TGTAAAACGACGGCCAGTAGCAAAAGCAGGAGT	Forward primer for NA Fragment 1
NA-F1-M13R	CAGGAAACAGCTATGACCCGACATGCTGAGCACTYCCTGAC	Reverse primer for NA Fragment 1
NA-F2-M13F	TGTAAAACGACGGCCAGTGAACTTGTRCAGTRGTAATG	Forward primer for NA Fragment 2
NA-F2-M13R	CAGGAAACAGCTATGACCAGTAGAAACAAGGAG	Reverse primer for NA Fragment 2
PB1-F1-M13F	TGTAAAACGACGGCCAGTAGCRAAAGCAGGCAAACCAT	Forward primer for PB1 Fragment 1
PB1-F1-M13R	CAGGAAACAGCTATGACCTGTTCAAGCTTTTCRCAWATGC	Reverse primer for PB1 Fragment 1
PB1-F2-M13F	TGTAAAACGACGGCCAGTCRATGACCAAAGATGCWGA	Forward primer for PB1 Fragment 2
PB1-F2-M13R	CAGGAAACAGCTATGACCAAGGTCATTGTTTATCATRTTG	Reverse primer for PB1 Fragment 2
PB1-F3-M13F	TGTAAAACGACGGCCAGTGTGGCYAATTTYAGCATGGAG	Forward primer for PB1 Fragment 3
PB1-F3-M13R	CAGGAAACAGCTATGACCAGTAGAAACAAGGCATTT	Reverse primer for PB1 Fragment 3