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. 2022 Oct 20;23(20):12610. doi: 10.3390/ijms232012610

Figure 1.

Figure 1

dNs treatment increased mtDNA copy number and alters global gene expression. (A) MtDNA copy number was increased in both control (CON) and ECHS1 KO cells after 8 days of dNs treatment. (B) Pearson correlation heatmap of transcriptome expression in CON cells and ECHS1 KO (KO) cells, either untreated (ut) or treated with dNs for 8 days. (C) Heatmap of 50 most significantly differentially expressed genes across all samples. The expression of mtDNA-encoded transcripts was increased in both CON and ECHS1 KO cells following dNs treatment. (D) Expression of mtDNA-encoded transcripts was increased following dNs treatment in both CON and ECHS1 KO cells. (E) Expression of nuclear-encoded OXPHOS complex I subunit genes were increased in both CON and ECHS1 KO cells following dNs treatment. Data shown is mean ± standard deviation (s.d.), with n ≥ 3. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to untreated (ut) CON cells; # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 compared to untreated (ut) ECHS1 KO cells (Student’s two-tailed t tests).