Figure 3.
OsCBL8 and OsCIPK17 can regulate seedling growth. (A,F) Morphological phenotypes of OsCBL8 and OsCIPK17 knockout and overexpression lines on rice seedling growth. Photographs of 14-day-old plants are shown. Bar = 2 cm. (B,C) Plant height and root length of OsCBL8 knockout and overexpression lines. Data are mean ± SD from 20 biological replicates for OsCBL8. (D,E) Fresh and dry weight of knockout and overexpression lines of OsCBL8 were measured. (G,H) Plant height and root length of OsCIPK17 knockout and overexpression lines. Data are mean ± SD from 12 biological replicates for OsCIPK17. Statistical analysis was performed using Šídák’s multiple comparisons test. (I,J) Fresh and dry weight of knockout and overexpression lines of OsCIPK17 were measured. Data are mean ± SD from three biological replicates. Statistical analysis was performed using Dunnett’s multiple comparisons test; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
