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. 2022 Oct 17;23(20):12395. doi: 10.3390/ijms232012395

Figure 3.

Figure 3

Expression of the MBW complex component genes in the “Buttercream” and the “Empress of India” nasturtium varieties and the amino acid sequence alignment of TmPAPs with Arabidopsis anthocyanin biosynthesis regulating R2R3 MYB proteins. (A) Expression of TmPAP1, TmPAP2, TmGL3, and TmTTG1 in the “Buttercream” and the “Empress of India” nasturtiums. RNA was isolated from 7-day-old soil-grown seedlings, and RT-PCR was used to examine the expression of the genes. The expression of TmACT2 was used as a control. (B) Amino acid alignment of TmPAPs with Arabidopsis PAP1, PAP2, MYB113, and MYB114. Underlines indicate the R2R3 domain, triangles indicate the amino acid required for the interaction of MYBs with bHLHs, and the star indicates the amino acid critical for DNA binding in GL1.