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. 2022 Oct 17;23(20):12395. doi: 10.3390/ijms232012395

Figure 6.

Figure 6

Subcellular localization and transcriptional activity of the nasturtium homologs of the Arabidopsis MBW complex proteins. (A) Subcellular localization of the nasturtium homologs of the Arabidopsis MBW complex proteins in Arabidopsis mesophyll protoplasts (with 400× magnificatio). Plasmids of constructs GFP-TmPAP1, GFP-TmPAP2, GFP-TmGL3, or GFP-TmTTG1 were co-transfected with the nucleus indicator construct NSL-RFP, respectively, into protoplasts, and pictures were taken after the transfected protoplasts were incubated for 20–22 h in darkness. (B) Transcriptional activity of the nasturtium homologs of the Arabidopsis MBW complex proteins. Plasmids of the effector constructs GD-TmPAP1, GD-TmPAP2, GD-TmGL3, GD-TmTTG1 or the control construct GD were co-transfected with the reporter construct GAL4:GUS, respectively, into the protoplasts, and GUS activity was assayed after the transfected protoplasts were incubated for 20–22 h in darkness. Data represent mean ± SD of three replicates. Significant differences from the GD control (* p < 0.001, ** p < 0.01).