TABLE 2.

List of functional genes that were found as the two gene clusters in the strain GO-4 genome and are responsible for biodegradation of 2-carboxybenzaldehyde via phthalic acid and protocatechuic acid

Gene, product	EC no.	COG category	aa length	Locus	IMG gene ID
phdK, 2-carboxybenzaldehyde dehydrogenase	1.2.1.78	C	492	4025393–4026871	2963527656
pht3, phthalate 4,5-dioxygenase oxygenase subunit	1.14.12.7	P	425	4023933–4025210	2963527655
pht2, phthalate 4,5-dioxygenase reductase subunit	1.14.12.7	C	316	4022846–4023796	2963527654
pht4, phthalate 4,5-cis-dihydrodiol dehydrogenase	1.3.1.64	R	382	4021639–4022862	2963527653
pht5, 4,5-dihydroxyphthalate decarboxylase	4.1.1.55		330	4020650–4021642	2963527652
pcaB, 3-carboxy-cis,cis-muconate cycloisomerase	5.5.1.2	F	445	1138719–1140056	2963525014
pcaF, acetyl-CoA C-acyltransferase	2.3.1.16	I	401	1140209–1141414	2963525015
pcaJ, 3-oxoadipate CoA-transferase beta subunit	2.8.3.6	I	257	1141438–1142211	2963525016
pcaI, 3-oxoadipate CoA-transferase alpha subunit	2.8.3.6	I	284	1142226–1143080	2963525017
pcaG, protocatechuate 3,4-dioxygenase alpha subunit	1.13.11.3	Q	195	1143223–1143810	2963525018
pcaH, protocatechuate 3,4-dioxygenase beta subunit	1.13.11.3	Q	233	1143814–1144515	2963525019
pcaC, 4-carboxymuconolactone decarboxylase	4.1.1.44	R	130	1144521–1144913	2963525020
pcaD, 3-oxoadipate enol-lactonase	3.1.1.24	H	266	1144906–1144706	2963525021