Figure 3.

Expression of basal markers in parent and As³⁺-exposed RT4 cells. (Ai–Aiii) TFAP2A expression, (Bi–Biii) P63 expression, (Ci–Ciii) TRIM29 expression, (Di–Diii) EGFR expression, and (Ei–Eiii) phosphorylation of EGFR in parent and 1 µM As³⁺-exposed RT4 cells at passages 1, 10, and 20 (P1, P10, and P20). Images are cropped to show relevant bands. Uncropped Western blots are shown in Supplementary Figure S2. Droplet digital PCR (ddPCR) analysis was performed to verify gene expression (Ai–Di). Western blot analysis was used to measure protein levels (Aii–Eii) and the integrated optical density (IOD) of each protein band was calculated (Aiii–Eiii). Ratio of pEGFR over total EGFR expression in parent and 1 μM As³⁺ RT4 cells. Gene expression is reported as copies per µL and protein expression was normalized to total protein per lane and then plotted as fold-change compared to the parent. Triplicate measurements of gene and protein data were performed and are reported as mean ± SEM. An unpaired t-test was performed. Asterisks indicate significant differences from the passaged-matched parent (p < 0.05).