FIG 7.

Binding characteristics of IR-specific monoclonal antibodies. Serially diluted preparations of the four affinity-purified IR-specific rMAbs cloned from rabbits immunized with recombinant VlsE were tested with ELISA against their respective IRs (1D11 against IR6; 15E2, 17A8, and 28D3 against IR4). The R package drc (Version 3.0-1) (79) was used to estimate the effective concentration and to plot the titration curves. EC50 (effective concentration at 50% of the maximum activity) values were estimated from the fitted curves, with a lower EC50 indicating stronger antigen affinity.