TABLE 2.
Specificity and sequences of IR-specific MAbs
| MAb | Specificity | EC50 (ng/mL)a | Variable heavy chain (VH) sequenceb | Variable light chain (VL) sequence |
|---|---|---|---|---|
| 1D11-4 | IR6 | 59.07 (1.75) | MGWSCIILFLVATATGVHSQSLVESGGGLVQPEGSLTLTCKASGFSFSSGYDMCWVRQAPGKGLEYIACIDAGDDITHYASWVKGRFTVSKTSSTTVTLQLNSLTVADTATYFCGRFWDLWGPGTLVTVSS (131 aa) | MGWSCIILFLVATATGVHSSVLTQTPSPVSAAVGGTVTINCQSSQSVYDSTWLGWYQQKPGQPPKLLIYKASNLASGVPSRFKGSGSGTHFTLTISDLECDDAATYYCVGGYSGSVDNWAFGGGTEVVVK (130 aa) |
| 15E2-1 | IR4 | 125.17 (5.10) | METGLRWLLLVAVLKGVQCQSLEESGGGLFKPTDTLTLTCTVSGIDLSSYAMIWVRQAPGKGLEWIGYIWSSGRIWYASWAKGRFTISRTSTTVDLKLASPTTEDTATYFCARLWDIWGPGTLVTVSS (128 aa) | MDTRAPTQLLGLLLLWLPGATFAQVLTQTPSSVSAAVGGTVTISCQASQSLYNGVNLAWYQQKPGQPPKLLIFGASNLESGVSSRFRGSGSGTQFTLTISGVQCDDAATYYCLGEFSCSSADCLAFGGGTEVVVK (135 aa) |
| 17A8-1 | IR4 | 86.14 (2.44) | METGLRWLLLVAVLKGVQCQSVEESGGRLVTPGTPLTLTCTVSGFPLSSYSMAWVRQAPGKGLEYIGFINTDGSAYYASWAKGRITISKTSTTVELKITSPTTEDTATYFCGTGNIWGPGTLVTVSS (127 aa) | MDTRAPTQLLGLLLLWLPGATFAQVLTQTPSSVSAAVGGTVTINCQASQSVSNNNVLAWFQQKPGQPPKRLIYSALTLDSGVPSRFKGSGSGTHFTLTISGVQCDDAATYYCAGGYDCSSNDCIAFGGGTEVVVK (135 aa) |
| 28D3-1 | IR4 | 245.91 (7.93) | METGLRWLLLVAVLKGVQCQSVEESGGRLVTPGTPLTLTCTVSGFSLSSYSMGWVRQAPGKGLEYIGMIISNNSTYYASWAKGRITISKTSTTVELKITSPTTEDTATYFCGTGNIWGPGTLVTVSS (127 aa) | MDTRAPTQLLGLLLLWLPGATFAQVLTQTPASVSAAVGGTVTINCQASQSTSNNNALAWFQQKPGQPPKRLIYSALTLDSGVPSRFKGSGSGTHFTLTISGVQCDDAATYYCAGGYDCSSNDCITFGGGTEVVVK (135 aa) |
a
EC50, effective concentration of 50% response level (and standard error) based on titration by ELISA. A lower EC50 indicates effective binding at a lower concentration (Fig. 7).
b
Peptide sequences from the topmost (or 2nd topmost) abundant sequences identified in the sorted single B cells producing IR-specific antibodies. The B cells originated from rabbits immunized with recombinant VlsE proteins (see Materials and Methods).