TABLE 3.
E. coli strains and plasmids used in this study
| Strain or plasmid | Description | Source |
|---|---|---|
| Strains | ||
| W1688 | l-Threonine-producing strain from E. coli MG1655 by mutation and molecular modification | CCTCC M2015233 |
| ΔycgF mutant | E. coli W1688 with deletion of ycgF | This study |
| ΔycgF nMagHigh mutant | ΔycgF strain harboring plasmid pET28a-EGFP-nMagHigh-OmpX | This study |
| ΔycgF pMagHigh mutant | ΔycgF strain harboring plasmid pET28a-mCherry-pMagHigh-OmpX | This study |
| T1 | This study | |
| Plasmids | ||
| pET28a-EGFP | Kanr, T7 promoter + EGFP | This study |
| pET28a-EGFP-nMagHigh | Kanr, T7 promoter + EGFP, trc promoter + nMagHigh-OmpX | This study |
| pET28a-mCherry | Kanr, T7 promoter + Cherry | This study |
| pET28a-mCherry-pMagHigh | Kanr, T7 promoter + mCherry, trc promoter + pMagHigh-OmpX | This study |
| pCas | Kanr, 30°C | This study |
| pTarget | AadAr, 37°C | This study |