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. 2022 Oct 15;23(20):12344. doi: 10.3390/ijms232012344

Figure 3.

Figure 3

ATP8B1 knockdown coincides with an increased mitochondrial ß-oxidation. (A) Oxygen consumption rate (OCR) in the absence of added substrate. Profiles shown are means +/− s.d. of 12–14 replicates of 2 independent experiments; (B) Quantification of ATP-coupled-, leak-associated and uncoupled OCR when no substrate was added. Data are extracted from the data shown in (A), corrected for non-mitochondrial OCR, and are expressed as means +/− s.d.; *** p < 0.0001 by multiple unpaired t-test; (C) Oxygen consumption rate (OCR) in the absence of added substrate with 3 µM etomoxir. Profiles shown are means +/− s.d. of 12–14 replicates of 2 independent experiments; (D,E) Quantification of basal (D) and maximal (E) respiration (corrected for non-mitochondrial respiration) in the absence of added substrate with/without etomoxir (Eto). Data are expressed as means +/− s.d. of 11–14 replicates from 2 independent experiments. **** p < 0.0001, ** p < 0.005 by 2way ANOVA with Tukey’s correction for multiple testing; ns, not significant.