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. 2022 Oct 15;23(20):12344. doi: 10.3390/ijms232012344

Figure 5.

Figure 5

ATP8B1 knockdown cells have increased mitochondrial phosphatidylethanolamine content and reduced phosphatidylethanolamine N-methyltransferase (PEMT) levels. (A) Mitochondrial lipid species expressed as percentage of total mitochondrial lipid. Data are expressed as mean +/− s.d. of 3 independent mitochondria isolations. Statistical analysis by multiple unpaired t-test; (B) Representative Western blot showing PEMT protein levels in total cell lysates of control and ATP8B1 knockdown HepG2 cells; (C) Densitometric analysis of PEMT levels; data are expressed as mean +/− s.d. of 6 replicates from 3 independent experiments. Statistical analysis by an unpaired t-test. *** p = 0.0001; (D) Confocal analysis of PEMT staining in red and nuclear dapi in blue. Bar = 20 nm; (E) PEMT mRNA expression in ATP8B1 knockdown HepG2 cells. Data were normalized to shControl cells and are expressed as means +/− s.e.m. of 15 replicates from 5 independent experiments. Statistical analysis by an unpaired t-test.