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Analysis of recombinant ASPH and ASPHmod. SDS-polyacrylamide electrophoresis of recombinant ASPH and ASPHmod proteins. (A) Western blot analysis. (B) Silver stain. PageRulerTM Plus Prestained Protein Ladder (5 µL; ThermoFisher Scientific, Waltham, MA, USA) provides the molecular weight standard.
