Table 1.
List of putative viruses discovered in this study and present in mite/mosquito hosts. Each viral sequence was compared with the NCBI non-redundant (nr) database using DIAMOND BLASTX. Hosts are represented with letters corresponding to mites (K), mite-free mosquitoes (M), and mite-detached mosquitoes (MK).
| Virus name | Contig name | Length | Provisional classification | Best hit in the NCBI/nr | Similarity | E-value | Host |
|---|---|---|---|---|---|---|---|
| Smedsang bunya-like virus | k119_16122 | 2,250 | Bunyavirales | BBQ05095.1 RNA-dependent RNA polymerase (Culex pseudovishnui bunya-like virus) | 34.5 | 1.34E−100 | K, M, and MK |
| Avesta bunya-like virus | k119_3430 | 1,925 | Bunyavirales | QNS17451.1 RNA-dependent RNA polymerase, partial (Serbia bunya-like virus 1) | 57.4 | 2.84E−249 | K, M, and MK |
| Heby virus | k119_4879 | 1,754 | Bunyavirales | QGA70945.1 RNA-dependent RNA polymerase (Salari virus) | 69 | 8.28E−286 | M and MK |
| Buska virus | k119_17401 | 7,663 | Bunyavirales | AJG39275.1 RNA-dependent RNA polymerase (Zhee mosquito virus) | 39.4 | 0 | K, M, and MK |
| Gaddsjo leishbunyavirus | k119_11873 | 2,442 |
Bunyavirales
/Leishbuviridae |
ANJ59510.1 putative RNA-dependent RNA polymerase (Leptomonas moramango leishbunyavirus) | 80.7 | 0 | M and MK |
| Sater virus | k119_979 | 13,942 |
Bunyavirales
/Nairo-like |
YP_009300680.1 RNA-dependent RNA polymerase (Shayang spider virus 1) | 25.4 | 1.42E−278 | K and MK |
| Fallet virus | k119_5606 | 7,027 |
Jingchuvirales
/Chuviridae |
API61887.1 RNA-directed RNA polymerase (Chuvirus Mos8Chu0) | 63 | 0 | K, M, and MK |
| Hede virus | k119_2521 | 1,378 |
Amarillovirales
/Flaviviridae |
YP_009179222.1 polyprotein (Xinzhou spider virus 2) | 31 | 2.07E−53 | K |
| Broddbo narna-like virus | k119_1307 | 3,122 |
Wolframvirales
/Narnaviridae |
APG77272.1 RNA-dependent RNA polymerase, partial (Wenling narna-like virus 6) | 35.9 | 6.23E−179 | K |
| Hytton narna-like virus | k119_17837 | 3,140 |
Wolframvirales/ Narnaviridae |
AGW51768.2 putative RNA-dependent RNA polymerase-like protein (Ochlerotatus-associated narna-like virus 2) | 73.4 | 0 | K, M, and MK |
| Hedemora virus | k119_6373 | 16,014 |
Mononegavirales/ Rhabdo-like |
YP_009304476.1 RNA-dependent RNA polymerase (Tacheng tick virus 7) | 29.2 | 5.70E−211 | K |
| Sonnboviken virus | k119_1814 | 1,016 |
Mononegavirales/ Lispi-like |
QMP82230.1 RNA-dependent RNA polymerase, partial (Megalopteran arli-related virus OKIAV106) | 25.2 | 2.02E−13 | K |
| Fors virus | k119_3330 | 3,921 |
Mononegavirales/ Lispi-like |
YP_009342285.1 RNA-dependent RNA polymerase (Wuchang romanomermis nematode virus 2) | 33.5 | 7.53E−171 | K |
| Osterbannback virus | k119_16137 | 13,129 | Mononegavirales | QRW42735.1 RNA-dependent RNA polymerase (Gordis virus) | 37.6 | 0 | K, M, and MK |
| Bro virus | k119_22347 | 6,397 |
Mononegavirales/ Xinmoviridae |
BBQ04817.1 RNA-dependent RNA polymerase (Culex tritaeniorhynchus anphevirus) | 41.2 | 0 | M |
| Malby virus | k119_10539 | 6,320 |
Mononegavirales/ Xinmoviridae |
BBQ04817.1 RNA-dependent RNA polymerase (Culex tritaeniorhynchus anphevirus) | 40 | 0 | K, M, and MK |
| Pelarsalen rhabdo-like virus | k119_4181 | 6,711 |
Mononegavirales/ Rhabdo-like |
QHA33680.1 RdRp (Atrato Rhabdo-like virus 3) | 48.6 | 0 | K, M, and MK |
| Tierp virus | k119_3941 | 2,428 |
Articulavirales/ Orthomyxoviridae |
QRW42655.1 polymerase PB1 (Usinis virus) | 57.6 | 4.35E−311 | K and MK |
| Husby virus | k119_19965 | 2,448 |
Articulavirales/ Orthomyxoviridae |
QGA70921.1 RNA-dependent RNA polymerase (Wuhan mosquito virus 4) | 55.9 | 3.01E−305 | K, M, and MK |
| Kagbo partiti-like virus | k119_14506 | 1,722 |
Durnavirales/ Partitiviridae |
APG78217.1 RdRp (Hubei partiti-like virus 22) | 60 | 2.19E−248 | MK |
| Ormpussen virus | k119_12042 | 1,630 |
Durnavirales/ Partitiviridae |
AWY11085.1 orf1 (galbut virus) | 36 | 8.71E−90 | K, M, and MK |
| Hebron partiti-like virus | k119_1779 | 1,699 |
Durnavirales/ Partitiviridae |
APG78260.1 RdRp (Hubei partiti-like virus 19) | 50.6 | 7.23E−194 | K, M, and MK |
| Hundmyran chaq-like virus | k119_19664 | 1,476 |
Durnavirales/ Partitiviridae |
AKH40308.1 orf1 (Chaq virus*) | 52.9 | 3.8E−103 | K, M, and MK |
| Nor picorna-like virus | k119_7745 | 9,260 |
Picornavirales/ Iflaviridae |
AWC26954.1 polyprotein (Culex picorna-like virus 1) | 53.9 | 0 | M |
| Dalkarlsbo virus | k119_10044 | 2,238 | Quenyaviridae | QIQ61196.1 putative RNA-dependent RNA polymerase (Nete virus) | 39.7 | 5.40E−158 | K, M, and MK |
| Morgongava virus | k119_3685 | 5,653 |
Muvirales/ Qinviridae |
QGA70948.1 RNA-dependent RNA polymerase (Vittskovle virus) | 33.9 | 3.85E−226 | K, M, and MK |
| Hallarsbo virus | k119_7784 | 4,121 |
Muvirales/ Qinviridae |
QLJ83493.1 RNA-dependent RNA polymerase (Fitzroy Crossing qinvirus 1) | 33 | 1.09E−222 | K, M, and MK |
| Berg reo-like virus | k119_12531 | 4,277 |
Reovirales/ Sedoreoviridae |
QHA33824.1 putative RdRp (Atrato reo-like virus) | 63.8 | 0 | M |
| Koversta virus | k119_2089 | 3,950 |
Ghabrivirales/ Totiviridae |
QHA33712.1 RdRp (Embera virus) | 54.2 | 0 | M |
| Disbo virus | k119_15173 | 2,401 |
Ghabrivirales/ Totiviridae |
YP_009552795.1 RNA-dependent RNA polymerase (Diatom colony-associated dsRNA virus 11) | 34.2 | 2.53E−131 | M |
| Karbo virus | k119_13033 | 1,727 |
Tymovirales/ Tymoviridae |
YP_009551972.1 polyprotein (Alfalfa virus F) | 44.5 | 5.20E−139 | M and MK |
| Ginka virga-like virus | k119_5852 | 2,312 |
Martellivirales/ Virgaviridae |
QHA33742.1 polyprotein (Atrato Virga-like virus 3) | 57.1 | 4.76E−285 | M and MK |
| Baggbo virus | k119_4924 | 4,407 | Unclassified Sobeliviralesa |
QIS87998.1 RNA-dependent RNA polymerase (Khabarov virus) | 42.7 | 1.87E−75 | K |
| Sala virus | k119_14050 | 5,220 | Unclassified Ellioviricetesa |
AGW51765.1 RNA-dependent RNA polymerase-like protein (uncultured virus) | 39.3 | 0 | K, M, and MK |
| Nedre virus | k119_4180 | 7,799 | Unclassified Ellioviricetesa |
AGW51765.1 RNA-dependent RNA polymerase-like protein (uncultured virus) | 39.2 | 0 | K and MK |
| Kvarnon virus | k119_3338 | 1,763 | Ortervirales/Metaviridae | QPF16710.1 putative RNA-dependent RNA polymerase (Aedes aegypti to virus 2) | 51.3 | 7.60E−194 | K, M, and MK |
| Fullsta virus | k119_9880 | 2,263 | Unclassified Pisuviricotaa |
QFR59041.1 putative RNA-dependent RNA polymerase, partial (Hanyang virus) | 41.5 | 2.57E−157 | K, M, and MK |
*
Chaq virus is often considered either a satellite virus or a segment or galbut virus.
a
Previously unclassified viruses annotated using RdRp-scan [1].