Table 1.
Kinetic parameters for the enzymatic activity of wild type EcoRK, A98G and A98G/I100V mutants towards D-ribose, D-arabinose and ATP (determination of activity was performed in three repeats).
| Vmax, μmol/min·mg | kcat, s−1 |
KM, mM |
|
|---|---|---|---|
| D-ribose: | |||
| Wild type | 340 ± 50 | 180 ± 30 | 0.15 ± 0.02 |
| A98G | 180 ± 20 | 97 ± 11 | 0.28 ± 0.03 |
| A98G/I100V | 130 ± 30 | 70 ± 16 | 0.24 ± 0.05 |
| D-arabinose: | |||
| Wild type | 3.6 ± 0.5 | 1.9 ± 0.3 | 470 ± 60 |
| A98G | 54 ± 5 | 29 ± 3 | 310 ± 30 |
| A98G/I100V | 33 ± 5 | 17 ± 3 | 730 ± 120 |
| ATP: | |||
| Wild type | 350 ± 40 | 185 ± 21 | 0.43 ± 0.05 |
| A98G | 160 ± 10 | 85 ± 5 | 0.58 ± 0.04 |
| A98G/I100V | 120 ± 10 | 63 ± 5 | 0.75 ± 0.09 |