Figure 3.

Induction of apoptosis by genistein at different concentrations in tumor MCF-7 cell lines in fluorescent microscope (magnification 400×). Cells were stained with annexin-Cy3.18 (AnnCy3) and 6-Carboxyfluorescein diacetate (6-CFDA). AnnCy3 binds to phosphatidylserine present in the outer leaflet of plasma membrane of cells starting the apoptotic process. The binding is observed as red fluorescence. 6-CFDA is used to measure viability. When this non-fluorescent compound enters living cells, esterases hydrolyze it, producing fluorescent compound 6-carboxyfluorescein (6-CF). This appears as green fluorescence. Cells cultured with 0.1% DMSO were considered as Control (+). Cells cultured with 15% DMSO were considered as Control (−).