Figure 4.

Lipid droplet formation induced by lipoprotein-deprived medium. Lung cancer cell lines are seeded into a lipoprotein-depleted medium. After seven days of growth, cells were fixed and stained with ORO and DAPI to stain LDs (labeled in red) and nuclei (labeled in blue), respectively. Images were captured at 400× magnification (scale bar, 50 µm) (A). The neutral lipid contents were quantitated using ImageJ as described previously (B).