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Restriction map and characteristics of subclones derived from pOT401. Restriction endonuclease sites are indicated above the top line. Clones were analyzed for expression of recombinant protein by Western immunoblot with the polyclonal antibody raised against whole-cell proteins of S. suis type 2 as the primary antibody. Subclones in which recombinant GDH was detected are denoted by a plus sign. Clones with no detectable recombinant GDH are denoted by a minus sign.
