Figure 2.

Impact of brlA or abaA disruption on radial growth and stress response of M. robertsii. (A,B) Images and diameters of fungal colonies grown at the optimal regime of 25 °C and L:D 12:12 for 7 days on nutrition-rich (PDA, SDAY, and 1/4 SDAY) and minimal (CDA) media. (C,D) Diameters of 7-day-old fungal colonies grown at the optimal regime on CDA amended with different amino acids as nitrogen sources. (E) Relative growth inhibition (RGI) percentages of fungal colonies grown at 25 °C for 7 days on CDA plates supplemented with H₂O₂ (2 mM), menadione (MND, 0.03 mM), calcofluor white (CFW, 15 μg/mL), and Congo red (CGR, 1 mg/mL) respectively. All colonies were initiated with hyphal mass discs (ϕ = 5 mm) attached to plates. p < 0.05 * or 0.001 *** in Tukey’s HSD tests (ns, no significance). Error bars: SDs from three independent replicates.