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Microglia depletion on P25. (A) The whole retinas of CX3CR1-GFP mice were collected on P19 and P25. CD31 was stained with red (n = 3 per group). Scale bar, 200 µm. MAX wave and OPS wave in ERG were tested on P19 (B) and P25 (C). (D) Immunofluorescence staining of CD31 (red), α-SMA (green), and DAPI (blue) on P25 in both Ctr OIR and PLX OIR groups. Scale bar, 100 µm (n = 3 per group). Bars = means ± SD; * p < 0.05; ** p < 0.01; **** p < 0.0001; NS, no significance.
