Figure 5.
The apoptosis declined after microglial depletion on P25. (A,B) Immunofluorescence staining of TUNEL (green) and DAPI (blue) on P25 in both the Ctr OIR and PLX OIR groups (n = 3 per group). Scale bar, 100 µm. (C) HE staining of retinas in the Ctr OIR and PLX OIR groups (n = 3 per group). Scale bar, 100 µm. (D,E) mRNA expression of apoptosis-associated genes were determined by qRT-PCR (n = 3 per group). Bars = means ± SD; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; NS, no significance.