TABLE 1.

Participation of cytoskeleton and metabolic activity of cells in attachment of trophozoites to Int-407 monolayer^a

Agents and concn	% Attachment after:
	Pretreatment of:		Treatment of cocultures
	G. lamblia	Int-407
Cytochalasin B (μg/ml)
2.5		82.8 ± 8
5	92 ± 9	85 ± 5
10	96 ± 3
Cytochalasin D (μg/ml)
2.5		89.7 ± 6
5	96 ± 4	78.1 ± 8
10	95.2 ± 8
EDTA (10 mM)	89.4 ± 3
Glutaraldehyde	0	28 ± 5
DMSO	100 ± 3	97 ± 5
Colchicine (mM)
2.5			42.1 ± 11
			23.2 ± 8
Mebendazole (μg/ml)
1			45.7 ± 7
0			32.2 ± 4
100			14 ± 2

^aAfter treatment of the cells, 10⁶ trophozoites were added to Int-407 monolayers in 24-well tissue culture plates for 2 h at 37°C, and unattached and adherent trophozoites suspensions were counted in a hemocytometer. See the text for different treatments. The results are expressed as a percentage of control values. Standard errors of mean were calculated from data of three experiments. P < 0.05 for all differences of treatment versus the control.