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The tested parameters and the levels set according to the chosen design.
| Factor | Levels | ||
|---|---|---|---|
| 1st | 2nd | 3rd | |
| A AgNO3 concentration M | 1 | 3 | 5 |
| B Extract:AgNO3 ratio | 1:9 | 5:5 | 9:1 |
| C pH | 3 | 5 | 8 |
| D Reaction temperature (℃) | 20 | 40 | 60 |
| E Reaction time (minutes) | 60 | 120 | 180 |
