Figure 1.

The expression of MED16 is associated with breast cancer tumorigenesis. (A,B) Gene expression data of MED16 in breast cancer and normal tissues from the TCGA and GEO databases showed that MED16 mRNA level was overexpressed in human breast cancer (C) RT-PCR was adopted to detect the mRNA expression level of MED16 in tumour and adjacent tissues (n = 25). (The values are compared to normal group and are represented as the means ± S.E.M (ns: not significant, ** p < 0.01). (D) MED16 expression in breast cancer subtypes from the TCGA cohort. (E,F) Western blot and RT-qPCR were used to detect the MED16 expression in different subtype BC cell lines. (G) IHC staining of MED16 expression in normal and ER+ BC tissues (the bar on the figure represents 50 µm), mostly located in the cytoplasm.