Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 20;12(10):1461. doi: 10.3390/life12101461

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Downregulation of MED16 reduces tamoxifen sensitivity in ER+ breast cancer cells. (A) Gene expression data of MED16 in T47D wild-type and tamoxifen-resistant T47D from GEO database (GSE125738). (B) 4OH-Tamoxifen sensitivity of tamoxifen-resistant T47D. (ns: not significant, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001) (C) Protein level of MED16 in WT and TAMR of T47D. (D) The MED16 protein expression levels of different time gradients at 1 μM 4OHT treatment. (E) Effect on control (vector) and MED16-OE, control (Parental) and MED16 knockout (KO1, KO2) MCF7 cells with different doses of 4OH-TAM at 72 h respectively. (F) Colony formation assay of untreated and treated with 4OHT (1 μM)in control (Parental) and MED16 knockout (KO2) MCF7 cells. (G) A micrograph showing mammosphere formation in control (Parental) and MED16 knockout (KO2) MCF7 cells. The length of bar represents 750 μm.