TABLE 1.
Oligonucleotide primers and LightCycler hybridization probes
| Target gene | GenBank accession no. | Primer | Nucleotide positions | Sequencea | Source |
|---|---|---|---|---|---|
| gyr | D10489 | gyrU | 219–239 | TTATGGTGCTGGGCAAATACA | 2 |
| gyrL | 536–556 | CACCATGTAAACCACCAGATA | 2 | ||
| gyrFL1 | 484–512 | ATTTTAACTGTTTTACATGCTGGTGGTAA-F | This study | ||
| gyrLC1 | 514–532 | Red640-TTTGGCGGTGGCGGATACA-ph | This study | ||
| hla | X01645 | hlaU | 498–518 | AGAAAATGGCATGCACAAAAA | 2 |
| hlaL | 874–895 | TATCAGTTGGGCTCTCTAAAA | 2 | ||
| hlaFL1 | 794–813 | CAGGAAAAATTGGCGGCCTT-F | This study | ||
| hlaLC1 | 815–836 | Red640-TTGGTGCAAATGTTTCGATTGG-ph | This study |
a
F, fluorescein; Red640, LightCycler-Red 640-N-hydroxysuccinimide ester; ph, 3′-phosphate.