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A. Overview of the SARS-COV-2 Spike S1 lineages in the Alpha, Delta, Omicron VOCs and six of the sewersheds with cryptic lineages. S1 amplifications were sequenced by subcloning (SC) and Sanger sequencing, or were sequenced using a PacBio (PB) deep sequencing. B. Plot of the number of synonymous and non-synonymous changes in the S1 sequences shown.
