Table 1.
Construction and genotypes of Fusarium strains used in this study.
| Strain Name | Construction (Plasmids Used for Transformation of Parental Strain) | Genotype | Strain Number |
|---|---|---|---|
| PH-1 | Wild-type (NRRL31084) | - | |
| PPKS2 | PH-1/pKR3 + pKR9 | pks15∆::loxP-(PPKI1HSV-TK hphTcbh2)-loxP | 1983 |
| PPKS4 | PH-1/pKR3+pKR9 (independent transformant) | pks15∆::loxP-(PPKI1HSV-TK hphTcbh2)-loxP | 1984 |
| IARS43 | PH-1/pRS107 | kmt6∆::loxP-(PtrpC nptII TtrpC)-loxP | 1288 |
| IARS45 | PH-1/pRS107 (independent transformant) | kmt6∆::loxP-(PtrpC nptII TtrpC)-loxP | 1289 |
| KPKS5 | PPKS4/pRS105+pRS106 | pks15∆::loxP-(PPKI1HSV-TK hphTcbh2)-loxP kmt6∆::loxP-(PtrpC nptII TtrpC)-loxP | 2025 |
| KPKS12 | PPKS4/pRS105+pRS106 | pks15∆::loxP-(PPKI1HSV-TK hphTcbh2)-loxP kmt6∆::loxP-(PtrpC nptII TtrpC)-loxP | 2026 |
| dTRI5#11 | PH-1 split marker 3.6 kb NotI-PstI from pGW859-12 (486 bp TRI5 5′ region with up-tag Not-SpeI in pGW859), 2.1 kb NotI-SacII from pGW860-19 (503 bp TRI5 3’ region with down-tag in pGW851) |
tri5Δ::loxP-(hph PXYN1-Cre)-loxP | 330 |
| dTRI5#11-19 | dTRI5#11 Cre-mediated popout (hygromycin sensitive) |
tri5Δ::loxP | 461 |
| IAG4_9 | dTRI5#11-19, split hph - PKS4 and PKS13 flanking regions | tri5Δ::loxP pks4,13:: loxP-(hph PXYN1-Cre)-loxP | 614 |
| IAG4_9_1 | IAG4_9 Cre-mediated popout, hygromycin sensitive | tri5Δ::loxP pks4,13:: loxP | 750 |
| IARS49 | IAG4_9_1/pRS105+pRS106 | tri5Δ::loxP pks4,13:: loxP kmt6∆::loxP-(PtrpC nptII TtrpC)-loxP | 1290 |
| IARS54 | IAG4_9_1/pRS105+pRS106 | tri5Δ::loxP pks4,13:: loxP kmt6∆::loxP-(PtrpC nptII TtrpC)-loxP | 1291 |
| TPKS2 | IAG4_9_1/pKR3+pKR9/popout | tri5Δ::loxP pks4,13:: loxP pks15∆::loxP | 2119 |
| TPKS4 | IAG4_9_1/pKR3+pKR9/popout | tri5Δ::loxP pks4,13:: loxP pks15∆::loxP | 2120 |
| KTPKS17 | TPKS4/pRS105+pRS106 | tri5Δ::loxP pks4,13:: loxP pks15∆::loxP-(PPKI1HSV-TK hphTcbh2)-loxP kmt6∆::loxP-(PtrpC nptII TtrpC)-loxP | 2263 |
| KTPKS38 | TPKS4/ pRS105+pRS106 | tri5Δ::loxP pks4,13:: loxP pks15∆::loxP-(PPKI1HSV-TK hphTcbh2)-loxP kmt6∆::loxP-(PtrpC nptII TtrpC)-loxP | 2266 |
| 7C PPKS3 | PPKS4 in locus complemented/additional silent HindIII in ORF | PKS15-C 7131T | 2284 |