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Quantification of F. keratoplasticum gene expression levels. (a) qPCR quantification of F. keratoplasticum erg6A, erg6B, cyp51A, cyp51B, cyp51C and abc1 mRNA expression levels (2−ΔCq) normalised with gpd1 and (b) Log2 fold increased expression (2−ΔΔCq) of logarithmic cells in response to 80 min exposure to VRC (16 mg/L), numbers on top of the bars indicate log2 fold changes. (c) Correlation between the log2 fold change values obtained using qPCR (Y-axis) and RNA-seq (X-axis).
